BI 399: Environmental Genomics Research
This course promises to be an engaging experience dedicated to exposing you to the most cutting edge molecular tools used to investigate microbial populations in their natural environments. You will apply genomics and bioinformatics tools to go from developing a research question to designing and carrying out a high throughput sequencing experiment to answer your research questions.
- Students will learn how to design proper high-throughput sequencing projects relevant to environmental microbiology.
- Students will learn the structural and functional importance of the rRNA operon and its utility in studying microbial diversity.
- Students will prepare bacterial and/or fungal sequencing libraries from DNA extracts by carrying out 16S rRNA and/or ITS library preparation using Illumina tag PCR, E-gel electrophoresis, DNA quantification, purification, and library quality checking.
- Students will also learn common issues associated with molecular microbial ecology approaches and troubleshooting options.
- Students will become familiar with the Linux and the command line, and will utilize QIIME to analyze high throughput sequence data.
- Students will learn how to properly select and utilize multivariate statistics to help answer their biological questions.
- Students will learn how to develop a scientific manuscript describing their research.